microcosmos
What Do These Algae Do With Four Genomes?
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Uploaded: | 2023-11-20 |
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If, for some reason, you ever find yourself reading a bunch of papers about cryptomonads, you might come across this strange fact: they have four genomes. That sounds like a lot of genomes. But what does that even mean? And what does the cryptomonas do with all those genomes?
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SOURCES:
http://e-algae.org/journal/view.php?doi=10.4490/algae.2013.28.4.307 ↩︎
https://doi.org/10.1093/gbe/evx123
https://www.nature.com/scitable/topicpage/the-origin-of-plastids-14125758/
https://linkinghub.elsevier.com/retrieve/pii/S1434461004701480
https://www.science.org/content/article/first-eukaryotes-found-without-normal-cellular-power-supply
https://academic.oup.com/gbe/article/doi/10.1093/gbe/evq082/573270?login=false
https://milnepublishing.geneseo.edu/botany/chapter/cryptophytes/
https://www.researchgate.net/publication/265520014_Cryptomonad_taxonomy_in_the_21st_century_The_first_200_years
https://www.google.com/books/edition/Algal_Culturing_Techniques/-qWHAwAAQBAJ?hl=en&gbpv=0
https://linkinghub.elsevier.com/retrieve/pii/S1434461004701315
http://www.schweizerbart.de/papers/nova_hedwigia/detail/79/73140/Pringsheim_s_living_legacy_CCALA_CCAP_SAG_and_UTEX?af=crossref
http://www.schweizerbart.de/papers/nova_hedwigia/detail/79/73140/Pringsheim_s_living_legacy_CCALA_CCAP_SAG_and_UTEX?af=crossref
https://linkinghub.elsevier.com/retrieve/pii/S096098222300458X
This video has been dubbed using an artificial voice via https://aloud.area120.google.com to increase accessibility. You can change the audio track language in the Settings menu.
Follow Journey to the Microcosmos:
Twitter: https://twitter.com/journeytomicro
Facebook: https://www.facebook.com/JourneyToMicro
Shop The Microcosmos:
https://www.microcosmos.store
Support the Microcosmos:
http://www.patreon.com/journeytomicro
More from Jam’s Germs:
Instagram: https://www.instagram.com/jam_and_germs
YouTube: https://www.youtube.com/channel/UCn4UedbiTeN96izf-CxEPbg
Hosted by Hank Green:
Twitter: https://twitter.com/hankgreen
YouTube: https://www.youtube.com/vlogbrothers
Music by Andrew Huang:
https://www.youtube.com/andrewhuang
Journey to the Microcosmos is a Complexly production.
Find out more at https://www.complexly.com
SOURCES:
http://e-algae.org/journal/view.php?doi=10.4490/algae.2013.28.4.307 ↩︎
https://doi.org/10.1093/gbe/evx123
https://www.nature.com/scitable/topicpage/the-origin-of-plastids-14125758/
https://linkinghub.elsevier.com/retrieve/pii/S1434461004701480
https://www.science.org/content/article/first-eukaryotes-found-without-normal-cellular-power-supply
https://academic.oup.com/gbe/article/doi/10.1093/gbe/evq082/573270?login=false
https://milnepublishing.geneseo.edu/botany/chapter/cryptophytes/
https://www.researchgate.net/publication/265520014_Cryptomonad_taxonomy_in_the_21st_century_The_first_200_years
https://www.google.com/books/edition/Algal_Culturing_Techniques/-qWHAwAAQBAJ?hl=en&gbpv=0
https://linkinghub.elsevier.com/retrieve/pii/S1434461004701315
http://www.schweizerbart.de/papers/nova_hedwigia/detail/79/73140/Pringsheim_s_living_legacy_CCALA_CCAP_SAG_and_UTEX?af=crossref
http://www.schweizerbart.de/papers/nova_hedwigia/detail/79/73140/Pringsheim_s_living_legacy_CCALA_CCAP_SAG_and_UTEX?af=crossref
https://linkinghub.elsevier.com/retrieve/pii/S096098222300458X
This video has been dubbed using an artificial voice via https://aloud.area120.google.com to increase accessibility. You can change the audio track language in the Settings menu.
If, for some reason, you ever find yourself reading a bunch of papers about cryptomonads, you might come across this strange fact: they have four genomes.
That sounds like a lot of genomes. But what exactly does that even mean?
And what does the cryptomonas do with all those genomes? The genus Cryptomonas was introduced in 1831 by the prolific microbiologist Christian Gottfried Ehrenberg. They’re mostly greenish brown in color, and in nature, green is often associated with photosynthesis and the various pigments that drive it.
And many cryptomonads are photosynthetic, using chlorophyll and accessory pigments that are packaged in photosynthetic organelles called plastids. But there are also several cryptomonad species that are no photosynthetic, like the colorless cryptomonas paramecium frantically dancing around the giant blepharisma in this clip. You might expect that this means the Cryptomonas paramecium doesn’t have plastids.
It isn’t performing photosynthesis, so what need would it have for a photosynthetic organelle? Well... Plenty, it turns out.
To understand why, we've got to look into how the cryptomonas got its plastid to begin with. In some ways, it’s a standard tale of endosymbiosis in the microcosmos. At some point in the past, an ancestor of cryptomonas consumed a red algae.
But instead of fully digesting it for food, the organism kept bits of the red algae around— namely, the algae’s plastid. Interestingly though, the red algae’s plastid is itself the product of endosymbiosis, a similar tale involving some ancient ancestor, only it had likely consumed a free-living cyanobacteria that it eventually converted into a plastid. So the red algae plastids are the product of what’s called primary endosymbiosis, and cryptomonad plastids are the result of what’s called secondary endosymbiosis.
And on top of co-opting the red algae’s plastid, the cryptomonas ancestor also took the red algae’s purple pigment phycoerythrin to power the photosynthesis. But plastids are not the only example of endosymbiosis contained in cryptomonads. The other one is a trait that cryptomonads share with us and almost all other eukaryotes: the mitochondrion.
And this is why the cryptomonad has four genomes. The first is its own nuclear DNA, the foundation of its identity. The second is its mitochondrial DNA, a remnant of DNA of an essential energy-producing organelle.
The third is the plastid DNA from the red algae. And the fourth? The fourth sequence of DNA is a weird one.
It’s called the nucleomorph, and it’s like a version of the red algae’s nucleus but just…less. This makes cryptomonads different from some other eukaryotes that have developed organelles through secondary endosymbiosis because those other organisms have managed to lose the nuclear remains of their endosymbiont altogether. And different is exciting when you are a scientist.
In this case, it makes cryptomonads useful to those who want to learn more about the diverse paths endosymbiosis takes as it converts organisms into organelles. Returning to our Cryptomonas paramecium, for example, scientists have looked into why the species would keep plastids around when they don’t use them for photosynthesis. But plastids are able to do more than just photosynthesis, it turns out.
At least, when scientists studied the plastids in Cryptomonas paramecium and several other non-photosynthetic cryptomonads, they found that the plastids were able to make fatty acids and amino acids and carry out a number of other important reactions, though actual functions a plastid might serve can vary from species to species. Despite their intrigue, cryptomonads are also challenging to study for a few reasons. For one, they can be a challenge to collect and keep alive.
James, our master of microscopes, said that they tend to die very quickly, seeming to disintegrate as soon as he looks at them under the microscope— though he has found that the non-photosynthetic ones are a little hardier. But in addition to the difficulty of keeping cryptomonads, they’re also really hard to tell apart using light microscopy. The features that distinguish species are just too muddied and difficult to categorize.
But microbiologists are resourceful, and since the end of the 19th century, they’ve been working to isolate and maintain pure cultures of algae that can provide scientists with the uncontaminated specimens they need to better understand the microbial world. One of these scientists was Ernst Georg Pringsheim, a German scientist who first began publishing papers on how to culture algae in 1912. He refined techniques that allowed him to pick up single cells with a pipette, and figured out the quality of water that would best allow his cultures to survive.
In 1922, he had moved to Prague to carry on his work as professor and develop his algal cultures. And while there, he met Olga Zimmerman, a student who would become his frequent collaborator. Pringsheim was offered a position that would allow him to return to Germany.
But he was Jewish, and Hitler was coming into power. So instead Pringsheim and his family left Prague for England in 1938, right before the German occupation of Czechoslovakia. And even with the turbulence of war around him, Pringsheim brought some of his algae strains with him to England.
And he maintained them, keeping them going so that when he did return to Germany in 1953, he could use them to establish cultures there as well. In fact, even after his death, Pringsheim’s cultures have served as the basis for more than 400 algae cultures distributed across collections maintained at various institutions around the world. And they’re still being used for research and teaching today.
Just this year, a group of scientists reported the results of their work studying a particular strain of cryptomonads whose origins lie in a Pringsheim culture that scientists have continued tending to for decades. When scientists looked inside this particular strain, they found two bacterial endosymbionts and a bacteriophage— or bacteria-infecting virus— also living inside the Cryptomonads. Their work revealed an expanded world within this particular strain. (And if you’re counting, this strain of Cryptomonas has seven genomes) We don’t know yet how the relationships between these organisms work, but the fact that this grouping has persisted through around 4000 generations of this strain suggests that it is probably very stable.
It is a little odd to describe the world within these strains as “stable” when the world that produced them was anything but. It’s also hard to adequately place meaning and value to Pringsheim’s commitment to his cultures in the context of the antisemitism and war that shaped his life and work. And yet it is a wonderful thing to leave a legacy built on care and curiosity.
It is a legacy that sustains itself by training the next generation in its methods, and providing the vessel for new questions. It leaves a mark on this world, as enduring, you might say, as an ancient genome buried in a cryptomonad. Thank you for coming on this journey with us as we explore the unseen world that surrounds us.
Don’t forget that you can now pick up a 2024 Microcosmos calendar, along with some other Complexly channel calendars like SciShow, and Eons, and Bizarre Beasts at complexlycalendars.com. There is a link down in the description, because look next year, you're going to need to know what day it is sometimes. The people on the screen right now, they are our Patreon patrons.
They allow us to continue exploring our world and to find strange, strange things about it, to share with you. And if you're interested in maybe becoming one of them, you can check out Patreon.com/JourneytoMicro. If you want to see more from our master of microscopes, James Weiss, you can check out Jam and Germs on Instagram.
And if you want to see more from us, there's always a subscribe button somewhere nearby.
That sounds like a lot of genomes. But what exactly does that even mean?
And what does the cryptomonas do with all those genomes? The genus Cryptomonas was introduced in 1831 by the prolific microbiologist Christian Gottfried Ehrenberg. They’re mostly greenish brown in color, and in nature, green is often associated with photosynthesis and the various pigments that drive it.
And many cryptomonads are photosynthetic, using chlorophyll and accessory pigments that are packaged in photosynthetic organelles called plastids. But there are also several cryptomonad species that are no photosynthetic, like the colorless cryptomonas paramecium frantically dancing around the giant blepharisma in this clip. You might expect that this means the Cryptomonas paramecium doesn’t have plastids.
It isn’t performing photosynthesis, so what need would it have for a photosynthetic organelle? Well... Plenty, it turns out.
To understand why, we've got to look into how the cryptomonas got its plastid to begin with. In some ways, it’s a standard tale of endosymbiosis in the microcosmos. At some point in the past, an ancestor of cryptomonas consumed a red algae.
But instead of fully digesting it for food, the organism kept bits of the red algae around— namely, the algae’s plastid. Interestingly though, the red algae’s plastid is itself the product of endosymbiosis, a similar tale involving some ancient ancestor, only it had likely consumed a free-living cyanobacteria that it eventually converted into a plastid. So the red algae plastids are the product of what’s called primary endosymbiosis, and cryptomonad plastids are the result of what’s called secondary endosymbiosis.
And on top of co-opting the red algae’s plastid, the cryptomonas ancestor also took the red algae’s purple pigment phycoerythrin to power the photosynthesis. But plastids are not the only example of endosymbiosis contained in cryptomonads. The other one is a trait that cryptomonads share with us and almost all other eukaryotes: the mitochondrion.
And this is why the cryptomonad has four genomes. The first is its own nuclear DNA, the foundation of its identity. The second is its mitochondrial DNA, a remnant of DNA of an essential energy-producing organelle.
The third is the plastid DNA from the red algae. And the fourth? The fourth sequence of DNA is a weird one.
It’s called the nucleomorph, and it’s like a version of the red algae’s nucleus but just…less. This makes cryptomonads different from some other eukaryotes that have developed organelles through secondary endosymbiosis because those other organisms have managed to lose the nuclear remains of their endosymbiont altogether. And different is exciting when you are a scientist.
In this case, it makes cryptomonads useful to those who want to learn more about the diverse paths endosymbiosis takes as it converts organisms into organelles. Returning to our Cryptomonas paramecium, for example, scientists have looked into why the species would keep plastids around when they don’t use them for photosynthesis. But plastids are able to do more than just photosynthesis, it turns out.
At least, when scientists studied the plastids in Cryptomonas paramecium and several other non-photosynthetic cryptomonads, they found that the plastids were able to make fatty acids and amino acids and carry out a number of other important reactions, though actual functions a plastid might serve can vary from species to species. Despite their intrigue, cryptomonads are also challenging to study for a few reasons. For one, they can be a challenge to collect and keep alive.
James, our master of microscopes, said that they tend to die very quickly, seeming to disintegrate as soon as he looks at them under the microscope— though he has found that the non-photosynthetic ones are a little hardier. But in addition to the difficulty of keeping cryptomonads, they’re also really hard to tell apart using light microscopy. The features that distinguish species are just too muddied and difficult to categorize.
But microbiologists are resourceful, and since the end of the 19th century, they’ve been working to isolate and maintain pure cultures of algae that can provide scientists with the uncontaminated specimens they need to better understand the microbial world. One of these scientists was Ernst Georg Pringsheim, a German scientist who first began publishing papers on how to culture algae in 1912. He refined techniques that allowed him to pick up single cells with a pipette, and figured out the quality of water that would best allow his cultures to survive.
In 1922, he had moved to Prague to carry on his work as professor and develop his algal cultures. And while there, he met Olga Zimmerman, a student who would become his frequent collaborator. Pringsheim was offered a position that would allow him to return to Germany.
But he was Jewish, and Hitler was coming into power. So instead Pringsheim and his family left Prague for England in 1938, right before the German occupation of Czechoslovakia. And even with the turbulence of war around him, Pringsheim brought some of his algae strains with him to England.
And he maintained them, keeping them going so that when he did return to Germany in 1953, he could use them to establish cultures there as well. In fact, even after his death, Pringsheim’s cultures have served as the basis for more than 400 algae cultures distributed across collections maintained at various institutions around the world. And they’re still being used for research and teaching today.
Just this year, a group of scientists reported the results of their work studying a particular strain of cryptomonads whose origins lie in a Pringsheim culture that scientists have continued tending to for decades. When scientists looked inside this particular strain, they found two bacterial endosymbionts and a bacteriophage— or bacteria-infecting virus— also living inside the Cryptomonads. Their work revealed an expanded world within this particular strain. (And if you’re counting, this strain of Cryptomonas has seven genomes) We don’t know yet how the relationships between these organisms work, but the fact that this grouping has persisted through around 4000 generations of this strain suggests that it is probably very stable.
It is a little odd to describe the world within these strains as “stable” when the world that produced them was anything but. It’s also hard to adequately place meaning and value to Pringsheim’s commitment to his cultures in the context of the antisemitism and war that shaped his life and work. And yet it is a wonderful thing to leave a legacy built on care and curiosity.
It is a legacy that sustains itself by training the next generation in its methods, and providing the vessel for new questions. It leaves a mark on this world, as enduring, you might say, as an ancient genome buried in a cryptomonad. Thank you for coming on this journey with us as we explore the unseen world that surrounds us.
Don’t forget that you can now pick up a 2024 Microcosmos calendar, along with some other Complexly channel calendars like SciShow, and Eons, and Bizarre Beasts at complexlycalendars.com. There is a link down in the description, because look next year, you're going to need to know what day it is sometimes. The people on the screen right now, they are our Patreon patrons.
They allow us to continue exploring our world and to find strange, strange things about it, to share with you. And if you're interested in maybe becoming one of them, you can check out Patreon.com/JourneytoMicro. If you want to see more from our master of microscopes, James Weiss, you can check out Jam and Germs on Instagram.
And if you want to see more from us, there's always a subscribe button somewhere nearby.